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Immunogenicity Prediction

T-cell activation assays

Robustness, sensitivity and specificity

Description

Immunogenicity of a protein reflects its capacity to induce specific activation of helper T-cells. These activated T-cells are then able to stimulate the production of protein-specific antibodies by B-cells.

The protein-specific activation of T-cells can be measured in vitro, allowing reliable evaluation of the intrinsic immunogenicity of a protein.

Our T-cell activation assays provide a robust, sensitive and highly specific approach to immunogenicity of your molecules such as proteins, peptides, hormones, antibodies....

 

Objectives:

  • Evaluate the specific immune response of human T-cells to your molecules
  • Test your molecules in a set of donors with diverse genetic backgrounds
  • Rank your molecules to immunogenic and non-immunogenic molecule controls
  • Enhance knowledge on your molecules and document your preclinical data
  • Rank candidates or versions of your molecules with respect to their immunogenicity to select the best candidate for clinic
  • Define immunodominant peptides for vaccine design

 

Features

The assay relies on antigen presenting cells and helper T-cells. The cells are collected from healthy donors with diverse HLA phenotype, representative of the majority of expressed phenotype in population.

The specific activation of T-cells directed against a molecule is measured by ELISpot (Enzyme linked ImmunoSpot assay). Non-molecule specific activation is measured for each healthy donor.

For each donor, controls include two reference proteins, respectively known to be immunogenic and non-immunogenic, allowing ranking of your protein to these reference proteins.

The analysis can be performed simultaneously for up to 5 proteins on the same set of donors.


Unique features of our T-cell activation assays:

  • High sensitivity
  • Control of the specificity of the immune response

Starting material

Depending on the size, we require 1 to 5mg of your protein.
The protein should be as pure as possible and free from any endotoxin.

Deliverables

A report including:

  • QC summary (including HLA phenotype and characterisation of donor cells)
  • The specific immune response of Human T-cells to your protein for a sample of donors
  • The specific immune response of same Human T-cells to immunogenic and non-immunogenic reference proteins
  • Ranking of all analysed proteins as highly, moderately, weakly or non-immunogenic